KCC2/SLC12A5-Specific Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85751-1-RR (same clone as 85751-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85751-1-RR (KCC2/SLC12A5-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85751-1-PBS in a different storage buffer formulation.

KCC2/SLC12A5-Specific Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 85751-1-PBS

Clone No.243141H8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, Indirect ELISA

Synonyms

KCC2, KCC2/SLC12A5, SLC12A5, Electroneutral potassium-chloride cotransporter 2, hKCC2

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Product Information

85751-1-PBS targets KCC2/SLC12A5-Specific in WB, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: solute carrier family 12 (potassium-chloride transporter), member 5
• Calculated Molecular Weight: 126 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: NM_020708
• Gene Symbol: KCC2
• Gene ID (NCBI): 57468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H2X9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases. This antibody can detect 130-150 kDa monomer and ~250 kDa dimer of KKC2 in brain lysate. (PMID: 23401525)