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Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue, mouse cerebellum tissue, rat cerebellum |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85751-1-RR targets KCC2/SLC12A5-Specific in WB, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | solute carrier family 12 (potassium-chloride transporter), member 5 |
| Calculated Molecular Weight | 126 kDa |
| Observed Molecular Weight | 130-150 kDa |
| GenBank Accession Number | NM_020708 |
| Gene Symbol | KCC2 |
| Gene ID (NCBI) | 57468 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H2X9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases. This antibody can detect 130-150 kDa monomer and ~250 kDa dimer of KKC2 in brain lysate. (PMID: 23401525)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KCC2/SLC12A5-Specific antibody 85751-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
