KCNJ15 Polyclonal antibody

• Featured Product
• KD/KO Validated

KCNJ15 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15988-1-AP

Synonyms

IRKK, KCNJ14, KCNJ15, KIR1.3, KIR4.2

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Validation Data Gallery

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  WB analysis of HeLa using 15988-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15988-1-AP (KCNJ15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15988-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15988-1-AP (KCNJ15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15988-1-AP

  IP result of anti-KCNJ15 (IP:15988-1-AP, 4ug; Detection:15988-1-AP 1:500) with HepG2 cells lysate 1000ug.

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  IHC staining of human liver using 15988-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15988-1-AP (KCNJ15 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15988-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15988-1-AP (KCNJ15 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

15988-1-AP targets KCNJ15 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KCNJ15 fusion protein Ag8795
• Full Name: potassium inwardly-rectifying channel, subfamily J, member 15
• Calculated Molecular Weight: 375 aa, 43 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC013327
• Gene Symbol: KCNJ15
• Gene ID (NCBI): 3772
• RRID: AB_2234166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCNJ15, also named as KIR1.3, KIR4.2, and IRKK, is a multi-pass membrane protein belonging to the inwardly-rectifying potassium channel (KIR) family. KIR channels perform functions as diverse as the regulation of resting membrane potential, maintenance of potassium ions homeostasis, control of heart rate and hormone secretion. Cloned from human kidney, the KCNJ15 gene is localized on chromosome 21 in the Down syndrome chromosome region 1 (DCR1) and has been identified as a type 2 diabetes-associated risk gene. The gene is most readily expressed in the pancreas and kidney and less in the lung. (PMID: 8995301; 9299242; 20085713)

Publications

Species	Application	Title
human	WB, IF	Nat Commun; KCNJ15/Kir4.2 couples with polyamines to sense weak extracellular electric fields in galvanotaxis.; Authors - Ken-Ichi Nakajima ; KD Validated
mouse	IHC	DNA Res; Identification of novel genes selectively expressed in the follicle-associated epithelium from the meta-analysis of transcriptomics data from multiple mouse cell and tissue populations.; Authors - Kobayashi Atsushi A
human	IHC	Ann Surg Oncol; KCNJ15 Expression and Malignant Behavior of Esophageal Squamous Cell Carcinoma.; Authors - Shunsuke Nakamura