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KCTD12 Polyclonal antibody, PBS Only (15523-1-PBS)

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WB analysis of mouse brain using 15523-1-AP (same clone as 15523-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 15523-1-AP (same clone as 15523-1-PBS)
Hela cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 15523-1-AP (same clone as 15523-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 15523-1-AP (same clone as 15523-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 15523-1-AP (same clone as 15523-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 15523-1-AP (same clone as 15523-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 15523-1-AP (same clone as 15523-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.
IHC staining of human bowen disease using 15523-1-AP (same clone as 15523-1-PBS)
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.
IHC staining of human bowen disease using 15523-1-AP (same clone as 15523-1-PBS)
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 15523-1-AP (same clone as 15523-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 15523-1-AP (same clone as 15523-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.
IHC staining of human intrahepatic cholangiocarcinoma using 15523-1-AP (same clone as 15523-1-PBS)
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.
IHC staining of human intrahepatic cholangiocarcinoma using 15523-1-AP (same clone as 15523-1-PBS)
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 15523-1-AP (same clone as 15523-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KCTD12 antibody (15523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 15523-1-AP (same clone as 15523-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using KCTD12 antibody (15523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

KCTD12 Polyclonal antibody, PBS Only

Name: KCTD12 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 15523-1-PBS

KCTD12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 15523-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

BTB/POZ domain-containing protein KCTD12, C13orf2, PFET1, Pfetin

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

15523-1-PBS targets KCTD12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5716 Product name: Recombinant human KCTD12 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-325 aa of BC013764 Sequence: MALADSTRGLPNGGGGGGGSGSSSSSAEPPLFPDIVELNVGGQVYVTRRCTVVSVPDSLLWRMFTQQQPQELARDSKGRFFLDRDGFLFRYILDYLRDLQLVLPDYFPERSRLQREAEYFELPELVRRLGAPQQPGPGPPPSRRGVHKEGSLGDELLPLGYSEPEQQEGASAGAPSPTLELASRSPSGGAAGPLLTPSQSLDGSRRSGYITIGYRGSYTIGRDAQADAKFRRVARITVCGKTSLAKEVFGDTLNESRDPDRPPERYTSRYYLKFNFLEQAFDKLSESGFHMVACSSTGTCAFASSTDQSEDKIWTSYTEYVFCRE Predict reactive species
Full Name	potassium channel tetramerisation domain containing 12
Calculated Molecular Weight	36 kDa
Observed Molecular Weight	36 kDa
GenBank Accession Number	BC013764
Gene Symbol	KCTD12
Gene ID (NCBI)	115207
RRID	AB_2132006
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96CX2
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

KCTD12 also known as PFET1 or PFETIN, which contains a voltage-gated potassium (K+) channel tetramerization T1 domain and a BTB/POZ (Bric-a-brac, Tram-track, Broad complex poxvirus and zinc finger) domain, belongs to the KCTD family and was initially identified in cochlea. In addition to being a K+ channel protein that responds to membrane potential7, KCTD12 also acts as an auxiliary subunit of GABAB (γ-aminobutyric acid type B) receptors, which regulate emotionality and neuronal excitability.