KCTD5 Recombinant antibody

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  WB analysis using 85210-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85210-5-RR (KCTD5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85210-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85210-5-RR against Human KCTD5 were performed. The affinity constant is 1.01 nM.

KCTD5 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85210-5-RR

Clone No.242864E2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

242864E2, BTB/POZ domain-containing protein KCTD5, KCTD17, KCTD2

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Tested Applications

• Positive WB detected in: HeLa cells, SW480 cells, A172 cells, RAW 264.7 cells, PC-12 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85210-5-RR targets KCTD5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KCTD5 fusion protein Ag7915
• Full Name: potassium channel tetramerisation domain containing 5
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC007314
• Gene Symbol: KCTD5
• Gene ID (NCBI): 54442
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NXV2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCTD5 belongs to the human potassium (K+) channel tetramerization domain (KCTD) family of proteins which share sequence similarity with the cytoplasmic domain of voltage-gated K+ channels (Kv channels). The KCTD proteins have relatively conserved N-terminal domains and variable C-termini (PMID: 24268103). KCTD5 interacts with cullin3 and has been proposed to function as substrate adapter for cullin3 based ubiquitin E3 ligases (PMID: 26188516).