KDM4A Polyclonal antibody

• Featured Product
• KD/KO Validated

KDM4A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, chIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24943-1-AP

Synonyms

JHDM3A, JMJD2, JMJD2A, KDM4A, KIAA0677, lysine (K) demethylase 4A, Lysine specific demethylase 4A

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Validation Data Gallery

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  WB analysis of MCF-7 using 24943-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24943-1-AP (KDM4A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 24943-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24943-1-AP (KDM4A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• ChIP: See 1 publications below

Product Information

The immunogen of 24943-1-AP is KDM4A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KDM4A fusion protein Ag20747
• Full Name: lysine (K)-specific demethylase 4A
• Calculated Molecular Weight: 1064 aa, 121 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC002558
• Gene Symbol: KDM4A
• Gene ID (NCBI): 9682
• RRID: AB_2879812
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KDM4A also named as JHDM3A, JMJD2, or KIAA0677 is a 1066 amino acid protein, which contains one JmjC domain, two Tudor domains and two PHD-type zinc fingers. KDM4A localizes in nucleus and belongs to the JHDM3 histone demethylase family. KDM4A is histone de-methylase that specifically demethylates Lys 9 and Lys 36 residues of Histone H3, thereby playing a central role in histone code. JMJD2A demethylation of lysine residues will generate formaldehyde and succinate. KDM4A also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. JMJD2A is a ubiquitously expressed nuclear protein. KDM4A exists as two isoforms. The short isoform of KDM4A in which the N-terminal demethylase domains has been deleted. The molecular weight of short isoform is 55Da.

Publications

Species	Application	Title
mouse	WB	Front Pharmacol; Folic Acid Attenuates Glial Activation in Neonatal Mice and Improves Adult Mood Disorders Through Epigenetic Regulation.; Authors - Tiantian Zhao
human	WB,chIP	Biochem Biophys Res Commun; The histone demethylase Kdm4 suppresses activation of hepatic stellate cell by inducing MiR-29 transcription.; Authors - Ming Kong ; KD Validated