CoraLite® Plus 488-conjugated KIAA0284 Monoclonal antibody

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  FC experiment of HeLa using CL488-66749

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human KIAA0284 (CL488-66749, Clone:1D4B8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

KIAA0284 Monoclonal Antibody for FC (Intra)

Cat No. CL488-66749

Clone No.1D4B8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Synonyms

Centrosomal protein 170B, Centrosomal protein of 170 kDa protein B, Cep170B, FAM68C, Protein KIAA0284

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66749 targets KIAA0284 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26852

  Product name: Recombinant human KIAA0284 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 525-881 aa of BC112928

  Sequence: LESPELSRASSATFRPVIRGDRDESDDGGVAQRMALLQEFASRPLGAAPQAEHQGLPVPGSPGGQKWVSRWASLADSYSDPGLTEDGLGRRGGEPEGSLPVRMRRRLPQLPSERADSPAGPESSRRSGPGPPELDSEQPSRLFGQEELDPDSLSDASGSDGGRGPEPGVEPQDSRRRSPQEGPTWSRGRRSPRAPGEPTPASFFIGDQNGDAVLSRKPLAAPGDGEGLGQTAQPSPPARDGVYVSANGRMVIQLRPGRSPEPDGPAPAFLRQESFTKEPASGPPAPGKPPHISSHPLLQDLAATRAARMDFHSQDTHLILKETETALAALEARLLSNSVDAECEGGSTPRPPEDALS

• Full Name: KIAA0284
• Calculated Molecular Weight: 1589 aa, 172 kDa
• Observed Molecular Weight: 172 kDa
• GenBank Accession Number: BC112928
• Gene Symbol: KIAA0284
• Gene ID (NCBI): 283638
• RRID: AB_3084270
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y4F5
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.