CoraLite® Plus 488-conjugated KIFC1 Recombinant monoclonal antibody

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  IF Staining of HeLa using CL488-85981

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 KIFC1 antibody (CL488-85981, Clone: 250588F10 ) at dilution of 1:600, CL594-phalloidin (red).

KIFC1 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-85981

Clone No.250588F10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

HSET, Kinesin-like protein 2, Kinesin-like protein KIFC1, Kinesin-related protein HSET, KNSL2

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-85981 targets KIFC1 in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag14492

  Product name: Recombinant human KIFC1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 382-725 aa of BC000712

  Sequence: GLLLFPSGPGGPSDPPTRLSLSRSDERRGTLSGAPAPPPRHDFSFDRVFPPGSGQDEVFEEIAMLVQSALDGYPVCIFAYGQTGSGKTFTMEGGPGGDPQLEGLIPRALRHLFSVAQELSGQGWTYSFVASYVEIYNETVRDLLATGTRKGQGGECEIRRAGPGSEELTVTNARYVPVSCEKEVDALLHLARQNRAVARTAQNERSSRSHSVFQLQISGEHSSRGLQCGAPLSLVDLAGSERLDPGLALGPGERERLRETQAINSSLSTLGLVIMALSNKESHVPYRNSKLTYLLQNSLGGSAKMLMFVNISPLEENVSESLNSLRFASKVNQCVIGTAQANRK

• Full Name: kinesin family member C1
• Calculated Molecular Weight: 673 aa, 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC000712
• Gene Symbol: KIFC1
• Gene ID (NCBI): 3833
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BW19
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KIFC1 is a molecular motor protein belonging to the kinesin-14 family that has motor domains at the C-terminus. KIFC1 is widely distributed, with highest expression in testis and participating in acrosome biogenesis and vesicle transport. It is also abundant in ovary, spleen, and liver. KIFC1 proteins are generally localized in the perinuclear region, and have been found to be involved chromosome alignment, congression and intracellular DNA transport (PMID: 12826589, PMID: 15236353, PMID: 23543461).