KISS1 Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• KISS1 Polyclonal antibody, PBS Only (18375-1-PBS)

• 

  WB analysis using 18375-1-AP (same clone as 18375-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18375-1-AP (KISS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 18375-1-AP (same clone as 18375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18375-1-AP (KISS1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 18375-1-AP (same clone as 18375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18375-1-AP (KISS1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18375-1-AP (same clone as 18375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18375-1-AP (KISS1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18375-1-AP (same clone as 18375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18375-1-AP (KISS1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 18375-1-AP (same clone as 18375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18375-1-AP (KISS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 18375-1-PBS

  Immunofluorescent analysis of NIH/3T3 cells using 18375-1-AP (KISS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

KISS1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 18375-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

KiSS 1, KiSS 1 metastasis suppressor, Kisspeptin 1, Kisspeptin-1, Metastasis suppressor KiSS 1

---

Product Information

18375-1-PBS targets KISS1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13240

  Product name: Recombinant human KISS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-138 aa of BC022819

  Sequence: MNSLVSWQLLLFLCATHFGEPLEKVASVGNSRPTGQQLESLGLLAPGEQSLPCTERKPAATARLSRRGTSLSPPPESSGSPQQPGLSAPHSRQIPAPQGAVLVQREKDLPNYNWNSFGLRFGKREAAPGNHGRSAGRG

• Full Name: KiSS-1 metastasis-suppressor
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC022819
• Gene Symbol: KISS1
• Gene ID (NCBI): 3814
• RRID: AB_11182705
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15726
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

KISS1, also known as kisspeptin, is a metastasis suppressor protein that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. It may regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A C-terminally amidated peptide of KISS1, metastin (kisspeptin-54 or kp-54), functions as the endogenous ligand of the G-protein coupled receptor GPR54 (PMID: 11457843; 11385580). KISS1-GPR54 signaling is involved in the regulation of reproduction, playing an important role in initiating secretion of gonadotropin-releasing hormone (GnRH) at puberty (PMID: 18753370).