KL Monoclonal antibody, PBS Only

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  WB analysis of mouse kidney using 67331-1-Ig (same clone as 67331-1-PBS)

  Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 67331-1-Ig (KL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67331-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 67331-1-Ig (same clone as 67331-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using KL antibody (67331-1-Ig, Clone: 4D1G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67331-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse kidney using 67331-1-Ig (same clone as 67331-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using KL antibody (67331-1-Ig, Clone: 4D1G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67331-1-PBS in a different storage buffer formulation.

KL Monoclonal Antibody for WB, IF-P, ELISA

Cat No. 67331-1-PBS

Clone No.4D1G11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

4D1G11, alpha klotho, klotho, α klotho, α-klotho

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Product Information

67331-1-PBS targets KL in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27838

  Product name: Recombinant human KL protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 34-184 aa of NM_004795

  Sequence: MEPGDGAQTWARFSRPPAPEAAGLFQGTFPDGFLWAVGSAAYQTEGGWQQHGKGASIWDTFTHHPLAPPGDSRNASLPLGAPSPLQPATGDVASDSYNNVFRDTEALRELGVTHYRFSISWARVLPNGSAGVPNREGLRYYRRLLERLRELG

• Full Name: klotho
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 125-135 kDa
• GenBank Accession Number: NM_004795
• Gene Symbol: KL
• Gene ID (NCBI): 9365
• RRID: AB_2882590
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UEF7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.