Tested Applications
| Positive WB detected in | mouse kidney tissue |
| Positive IF-P detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67331-1-Ig targets KL in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27838 Product name: Recombinant human KL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 34-184 aa of NM_004795 Sequence: MEPGDGAQTWARFSRPPAPEAAGLFQGTFPDGFLWAVGSAAYQTEGGWQQHGKGASIWDTFTHHPLAPPGDSRNASLPLGAPSPLQPATGDVASDSYNNVFRDTEALRELGVTHYRFSISWARVLPNGSAGVPNREGLRYYRRLLERLRELG Predict reactive species |
| Full Name | klotho |
| Calculated Molecular Weight | 116 kDa |
| Observed Molecular Weight | 125-135 kDa |
| GenBank Accession Number | NM_004795 |
| Gene Symbol | KL |
| Gene ID (NCBI) | 9365 |
| RRID | AB_2882590 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UEF7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KL antibody 67331-1-Ig | Download protocol |
| WB protocol for KL antibody 67331-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KL monoclonal antibody (catalog 67331-1-Ig) has 3 citations total.
The publications appear in the journals Apoptosis, Frontiers in Medicine (Lausanne), and Toxicology Letters.
The research fields covered include diabetic kidney disease and ferroptosis, cellular senescence, and uremic cardiomyopathy. Applications cited include immunofluorescence (IF) in mouse, rat, and human samples.
| Species | Application | Title |
|---|---|---|
Apoptosis Vitamin D inhibits ferroptosis and mitigates the kidney injury of prediabetic mice by activating the Klotho/p53 signaling pathway | ||
Front Med (Lausanne) Shenyuan granules improve cellular senescence through Klotho-mediated p16/p21 signaling pathway in diabetic kidney disease | ||
Toxicol Lett Damage of uremic myocardium by p-cresyl sulfate and the ameliorative effect of Klotho by regulating SIRT6 ubiquitination. |
