KLHL25 Monoclonal antibody

KLHL25 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D8B8

Cat no : 67774-1-Ig

Synonyms

ENC 2, ENC2, FLJ12587, FLJ45015, kelch like 25 (Drosophila), Kelch like protein 25, KLHL25

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Validation Data Gallery

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  WB analysis using 67774-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67774-1-Ig (KLHL25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 67774-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67774-1-Ig (KLHL25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 67774-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67774-1-Ig (KLHL25 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 67774-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67774-1-Ig (KLHL25 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HT-29 cells, HepG2 cells, NCCIT cells, MOLT-4 cells, Raji cells, Ramos cells, Daudi cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67774-1-Ig targets KLHL25 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KLHL25 fusion protein Ag27037
• Full Name: kelch-like 25 (Drosophila)
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC028100
• Gene Symbol: KLHL25
• Gene ID (NCBI): 64410
• RRID: AB_2918539
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.