CoraLite®594-conjugated KMO Monoclonal antibody

KMO Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D2F2

Cat no : CL594-60029

Synonyms

dJ317G22.1, KMO, Kynurenine 3 hydroxylase, Kynurenine 3 monooxygenase

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Validation Data Gallery

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  IF Staining of C6 using CL594-60029

  Immunofluorescent analysis of (4% PFA) fixed C6 cells using CoraLite®594 KMO antibody (CL594-60029, Clone: 2D2F2 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: C6 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60029 targets KMO in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KMO fusion protein Ag1109
• Full Name: kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
• Calculated Molecular Weight: 486 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC005297
• Gene Symbol: KMO
• Gene ID (NCBI): 8564
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KMO(Kynurenine 3-monooxygenase) is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).