KRAS-2A-specific Polyclonal antibody
KRAS-2A-specific Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 16156-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | NIH/3T3 cells |
Positive IF/ICC detected in | SH-SY5Y cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
Product Information
16156-1-AP targets KRAS-2A-specific in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
Calculated Molecular Weight | 21 kDa |
Observed Molecular Weight | 21-24 kDa |
GenBank Accession Number | NM_033360 |
Gene Symbol | KRAS |
Gene ID (NCBI) | 3845 |
RRID | AB_2234477 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 2A and 2B. This antibody is specific for KRAS-2A, which also named as KRAS-4A .
Protocols
Product Specific Protocols | |
---|---|
WB protocol for KRAS-2A-specific antibody 16156-1-AP | Download protocol |
IF protocol for KRAS-2A-specific antibody 16156-1-AP | Download protocol |
FC protocol for KRAS-2A-specific antibody 16156-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Chem Biol Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System. | ||
Sci Signal Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies. | ||
Life Sci Alliance A new ferrocene derivative blocks K-Ras localization and function by oxidative modification at His95 |