Filter: AllIHC

IHC staining of human oesophagus cancer using Biotin-16835
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using Biotin-16835
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using Biotin-16835
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using Biotin-16835
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using Biotin-16835
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using Biotin-16835
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using Biotin-16835
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

Biotin-conjugated Cytokeratin 80 Polyclonal antibody

Name: Biotin-conjugated Cytokeratin 80 Polyclonal antibody
Brand: Proteintech
SKU: Biotin-16835

Cytokeratin 80 Polyclonal Antibody for IHC

Cat No. Biotin-16835

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Synonyms

KRT80, CK 80, CK-80, Cytokeratin-80, K80

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Biotin

Unconjugated Biotin

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in

human oesophagus cancer tissue, human colon cancer, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-16835 targets Cytokeratin 80 in IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10498 Product name: Recombinant human KRT80 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-422 aa of BC065180 Sequence: MACRSCVVGFSSLSSCEVTPVGSPRPGTSGWDSCRAPGPGFSSRSLTGCWSAGTISKVTVNPGLLVPLDVKLDPAVQQLKNQEKEEMKALNDKFASLIGKVQALEQRNQLLETRWSFLQGQDSAIFDLGHLYEEYQGRLQEELRKVSQERGQLEANLLQVLEKVEEFRIRYEDEISKRTDMEFTFVQLKKDLDAECLHRTELETKLKSLESFVELMKTIYEQELKDLAAQVKDVSVTVGMDSRCHIDLSGIVEEVKAQYDAVAARSLEEAEAYSRSQLEEQAARSAEYGSSLQSSRSEIADLNVRIQKLRSQILSVKSHCLKLEENIKTAEEQGELAFQDAKTKLAQLEAALQQAKQDMARQLRKYQELMNVKLALDIEIATYRKLVEGEEGRMDSPSATVVSAVQSRCKTAPSLPYPLCSL Predict reactive species
Full Name	keratin 80
Calculated Molecular Weight	422 aa, 47 kDa
Observed Molecular Weight	47 kDa, 50 kDa
GenBank Accession Number	BC065180
Gene Symbol	KRT80
Gene ID (NCBI)	144501
RRID	AB_2934330
Conjugate	Biotin
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6KB66
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.