FITC Plus Anti-Mouse Ki-67 Rabbit Recombinant Antibody

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  FC experiment of C57BL/6 mouse splenocytes using FITC-98224

  1x10^6 untreated or Con A treated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug FITC Plus Anti-Mouse Ki-67 Rabbit RecAb (FITC-98224, Clone: 241768E2). Cells were co-stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of C57BL/6 mouse splenocytes using FITC-98224

  1x10^6 Con A treated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug FITC Plus Anti-Mouse Ki-67 Rabbit RecAb (FITC-98224, Clone: 241768E2) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). 1x10^6 untreated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug FITC Plus Anti-Mouse Ki-67 Rabbit RecAb (FITC-98224, Clone:241768E2) (black, dashed). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Ki-67 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. FITC-98224

Clone No.241768E2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Ki 67, Ki67, Mki67, 241768E2, Proliferation related Ki 67 antigen

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Tested Applications

• Positive FC (Intra) detected in: Con A treated C57BL/6 mouse splenocytes

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98224 targets Ki-67 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Ki-67 fusion protein Eg1548
• Full Name: antigen identified by monoclonal antibody Ki 67
• Calculated Molecular Weight: 351kDa
• GenBank Accession Number: NM_001081117.2
• Gene Symbol: ki67
• Gene ID (NCBI): 17345
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: E9PVX6-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki-67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.