L-VOCC Polyclonal antibody

L-VOCC Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

16

Reviews (1)

Cat no : 21774-1-AP

Synonyms

CACH2, CACN2, CACNA1C, CACNL1A1, CaV1.2, CCHL1A1, L VOCC, TS

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Validation Data Gallery

• 

  IHC staining of mouse brain using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21774-1-AP (L-VOCC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21774-1-AP (L-VOCC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21774-1-AP (L-VOCC Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat brain using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21774-1-AP (L-VOCC Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse liver using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 21774-1-AP (L-VOCC antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 21774-1-AP (L-VOCC antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 21774-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L-VOCC antibody (21774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 21774-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L-VOCC antibody (21774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse brain tissue, rat brain tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 14 publications below
• IF: See 3 publications below

Product Information

The immunogen of 21774-1-AP is L-VOCC Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: L-VOCC fusion protein Ag16455
• Full Name: calcium channel, voltage-dependent, L type, alpha 1C subunit
• Calculated molecular weight: 2135 aa, 239 kDa
• GenBank accession number: BC146846
• Gene symbol: CACNA1C
• Gene ID (NCBI): 775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	WB	Antioxid Redox Signal; Anthracycline-induced atrial structural and electrical remodeling characterizes early cardiotoxicity and contributes to atrial conductive instability and dysfunction.; Authors - Ruopeng Tan
rat	WB	Front Immunol; Interleukin-6-Mediated-Ca2+ Handling Abnormalities Contributes to Atrial Fibrillation in Sterile Pericarditis Rats.; Authors - Jie Liao
rat	WB	Mol Nutr Food Res; Quercetin Attenuates Ethanol-Induced Iron Uptake and Myocardial Injury by Regulating the Angiotensin II-L-Type Calcium Channel.; Authors - Xiaoping Guo
human,mouse	WB	Commun Biol; Cytosolic peptides encoding CaV1 C-termini downregulate the calcium channel activity-neuritogenesis coupling.; Authors - Yaxiong Yang
rat	WB,IF	J Transl Med; Harm of circadian misalignment to the hearts of the adolescent wistar rats.; Authors - YunLei Wang
rabbit	WB	Cardiovasc Drugs Ther; Effects of Angiotensin Receptor-Neprilysin Inhibitor in Arrhythmogenicity Following Left Atrial Appendage Closure in an Animal Model.; Authors - Wen-Han Cheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wu (Verified Customer) (07-16-2021)

Works good for IHC assay

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: glandular lumen

L-VOCC Antibody Immunohistochemistry validation (1:100 dilution) in glandular lumen (Cat no:21774-1-AP)

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