CoraLite® Plus 488-conjugated L-VOCC Polyclonal antibody

L-VOCC Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21774

Synonyms

CACH2, CACN2, CACNA1C, CACNL1A1, CaV1.2, CCHL1A1, L VOCC, TS

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Validation Data Gallery

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  FC experiment of HeLa using CL488-21774

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human L-VOCC (CL488-21774) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using CL488-21774

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human L-VOCC (CL488-21774) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-21774 targets L-VOCC in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: L-VOCC fusion protein Ag16455
• Full Name: calcium channel, voltage-dependent, L type, alpha 1C subunit
• Calculated Molecular Weight: 2135 aa, 239 kDa
• GenBank Accession Number: BC146846
• Gene Symbol: CACNA1C
• Gene ID (NCBI): 775
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.