CoraLite® Plus 488-conjugated LAIR1 Recombinant antibody

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  IF Staining of THP-1 using CL488-84677-4

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 488 LAIR1 antibody (CL488-84677-4, Clone: 241805C2 ) at dilution of 1:200.

LAIR1 Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-84677-4

Clone No.241805C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

241805C2, CD305, hLAIR1, LAIR 1, LAIR-1

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Tested Applications

• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84677-4 targets LAIR1 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LAIR1 fusion protein Eg2329
• Full Name: leukocyte-associated immunoglobulin-like receptor 1
• Calculated Molecular Weight: 287 aa, 31 kDa
• GenBank Accession Number: BC027899
• Gene Symbol: LAIR1
• Gene ID (NCBI): 3903
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6GTX8
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs (PMID: 9285412). LAIR1 is expressed on the majority of human PBMCs, including NK, T, B, monocytes, and dendritic cells, as well as the majority of thymocytes (PMID: 10229813). It functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of NK cells, B cells and T cells. LAIR1 is a collagen receptor (PMID: 16754721). Tumor-expressed collagens can modulate immune cell function through the inhibitory collagen receptor LAIR1 (PMID: 21955987).