LAMA3 Recombinant monoclonal antibody

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  WB analysis using 83210-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83210-2-RR (LAMA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83210-2-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83210-2-RR (LAMA3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LAMA3 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 83210-2-RR

Clone No.230443C1

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

230443C1, BM600, E170, lama3a, Laminin subunit alpha 3

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, NCI-H1299 cells, HaCaT cells, A549 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

83210-2-RR targets LAMA3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: laminin, alpha 3
• Calculated Molecular Weight: 367 kDa
• Observed Molecular Weight: 260 kDa
• GenBank Accession Number: NM_000227
• Gene Symbol: LAMA3
• Gene ID (NCBI): 3909
• RRID: AB_3670895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16787
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Laminin subunit alpha 3 (LAMA3) expression is mediated by an epithelial enhancer via a synergistic effect of AP-1 binding sites.10 LAMA3 has been implicated in the invasion and progression of tumor cells and encodes for the α3 chain of laminin-5 (α3, β3, γ2, 332). Its dysfunction manifests as a blistering skin disease similar to junctional epidermolysis bullosa in humans. (PMID: 31557618)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Rep; LAMA3 overexpression enhances proliferation, migration and invasion in esophageal squamous cell carcinoma based on bioinformatics and experimental validation; Authors - Xia Wu ; KD Validated