Tested Applications
Positive WB detected in | A431 cells, U2OS cells, rat lung tissue |
Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:750-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
85055-4-RR targets LAMB3 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
Tested Reactivity | human, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | LAMB3 fusion protein Ag25192 Predict reactive species |
Full Name | laminin, beta 3 |
Calculated Molecular Weight | 1172 aa, 130 kDa |
Observed Molecular Weight | 130-140 kDa |
GenBank Accession Number | BC075838 |
Gene Symbol | LAMB3 |
Gene ID (NCBI) | 3914 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13751 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LAMB3 (β3 subunit of laminin) is an important extracellular matrix protein, which plays an important role in cell adhesion, migration, signal transduction and the formation and stability of basement membrane. LAMB3 plays a key role in the basement membrane of skin and mucosa, maintaining the adhesion between epidermis and dermis. Its abnormal function will lead to skin bullous disease (such as severe borderline epidermolaxis, sJEB), which is characterized by blisters and ulcers on skin and mucosa. LAMB3 is highly expressed in many cancers, including pancreatic ductal adenocarcinoma (PDAC), lung cancer, head and neck squamous cell carcinoma, etc., which is closely related to the proliferation, invasion and metastasis of tumor cells.
Protocols
Product Specific Protocols | |
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WB protocol for LAMB3 antibody 85055-4-RR | Download protocol |
IHC protocol for LAMB3 antibody 85055-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |