LAMB3 Recombinant antibody

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  WB analysis using 85055-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85055-4-RR (LAMB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 85055-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85055-4-RR (LAMB3 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85055-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85055-4-RR against Human LAMB3 were performed. The affinity constant is 0.244 nM.

LAMB3 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 85055-4-RR

Clone No.242442A8

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

Laminin B1k chain, 242442A8, Epiligrin subunit bata, Kalinin B1 chain, Kalinin subunit beta

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Tested Applications

• Positive WB detected in: A431 cells, U2OS cells, rat lung tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85055-4-RR targets LAMB3 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LAMB3 fusion protein Ag25192
• Full Name: laminin, beta 3
• Calculated Molecular Weight: 1172 aa, 130 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC075838
• Gene Symbol: LAMB3
• Gene ID (NCBI): 3914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13751
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAMB3 (β3 subunit of laminin) is an important extracellular matrix protein, which plays an important role in cell adhesion, migration, signal transduction and the formation and stability of basement membrane. LAMB3 plays a key role in the basement membrane of skin and mucosa, maintaining the adhesion between epidermis and dermis. Its abnormal function will lead to skin bullous disease (such as severe borderline epidermolaxis, sJEB), which is characterized by blisters and ulcers on skin and mucosa. LAMB3 is highly expressed in many cancers, including pancreatic ductal adenocarcinoma (PDAC), lung cancer, head and neck squamous cell carcinoma, etc., which is closely related to the proliferation, invasion and metastasis of tumor cells.