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LDAH Polyclonal antibody, PBS Only (31714-1-PBS)

LDAH Polyclonal antibody, PBS Only

Name: LDAH Polyclonal antibody, PBS Only,
Brand: Proteintech

LDAH Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 31714-1-PBS

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C2orf43, HLDAH, Lipid Droplet Associated Hydrolase, UPF0554 protein C2orf43

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis using 31714-1-AP (same clone as 31714-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 31714-1-AP (C2orf43 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31714-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 31714-1-AP (same clone as 31714-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31714-1-AP (LDAH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31714-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 31714-1-AP (same clone as 31714-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using LDAH antibody (31714-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31714-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information

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Product Information

31714-1-PBS targets LDAH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag36372 Product name: Recombinant human C2orf43 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 100-325 aa of BC017473 Sequence: DSNAQEIKDIYGLNGQIEHKLAFLRTHVPKDMKLVLIGHSIGSYFTLQMLKRVPELPVIRAFLLFPTIERMSESPNGRIATPLLCWFRYVLYVTGYLLLKPCPETIKSLLIRRGLQVMNLENEFSPLNILEPFCLANAAYLGGQEMMEVVKRDDETIKEHLCKLTFYYGTIDPWCPKEYYEDIKKDFPEGDIRLCEKNIPHAFITHFNQEMADMIADSLKDDLSKM Predict reactive species
Full Name	chromosome 2 open reading frame 43
Observed Molecular Weight	32 kDa
GenBank Accession Number	BC017473
Gene Symbol	C2orf43
Gene ID (NCBI)	60526
RRID	AB_3670093
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	Q9H6V9
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

LDAH, also known as C2orf43, is a newly identified LD protein. It has 4 isoforms and is abundantly expressed in tissues that predominantly store triacylglycerol (TAG), including liver and white and brown adipose tissue (PMID: 24357060).