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LDHC-Specific Polyclonal antibody, PBS Only (19989-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis of human testis using 19989-1-AP (same clone as 19989-1-PBS)
human testis tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.
WB analysis using 19989-1-AP (same clone as 19989-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 19989-1-AP (same clone as 19989-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.
WB analysis of mouse lung using 19989-1-AP (same clone as 19989-1-PBS)
mouse lung tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 19989-1-AP (same clone as 19989-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 19989-1-AP (same clone as 19989-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.
IP experiment of mouse testis using 19989-1-AP (same clone as 19989-1-PBS)
IP result of anti-LDHC-Specific (IP:19989-1-AP, 4ug; Detection:19989-1-AP 1:500) with mouse testis tissue lysate 2400ug. This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 19989-1-AP (same clone as 19989-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 19989-1-AP (same clone as 19989-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 19989-1-AP (same clone as 19989-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 19989-1-AP (same clone as 19989-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 19989-1-AP (same clone as 19989-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 19989-1-AP (same clone as 19989-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 19989-1-AP (same clone as 19989-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 19989-1-AP (same clone as 19989-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 19989-1-AP (same clone as 19989-1-PBS)
Immunofluorescent analysis of Hela cells, using LDHC antibody 19989-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

LDHC-Specific Polyclonal antibody, PBS Only

Name: LDHC-Specific Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 19989-1-PBS

LDHC-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 19989-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

19989-1-PBS targets LDHC-Specific in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	lactate dehydrogenase C
Calculated Molecular Weight	36 kDa
Observed Molecular Weight	30-36 kDa
GenBank Accession Number	NM_002301
Gene Symbol	LDHC
Gene ID (NCBI)	3948
RRID	AB_10637847
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P07864
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

LDHC, also named as CT32 and LDH-X, belongs to the LDH/MDH superfamily and LDH family. LDHC is a possible role in sperm motility. LDHC catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. LDHC is a testis-specific isoform. Abnormal LDHC expression is associated with several forms of cancer. The antibody has no cross reaction with LDHA and LDHB.