LGR6 Polyclonal antibody

• Featured Product
• KD/KO Validated

LGR6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 17658-1-AP

Synonyms

GPCR, LGR6, VTS20631

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Validation Data Gallery

• 

  WB analysis of HUVEC using 17658-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 17658-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17658-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17658-1-AP (LGR6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17658-1-AP (LGR6 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HUVEC cells, mouse testis tissue, human brain tissue
• Positive IHC detected in: human testis tissue, human spleen tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• ELISA: See 1 publications below

Product Information

17658-1-AP targets LGR6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LGR6 fusion protein Ag11909
• Full Name: leucine-rich repeat-containing G protein-coupled receptor 6
• Calculated Molecular Weight: 915 aa, 99 kDa
• Observed Molecular Weight: 104 kDa
• GenBank Accession Number: BC047905
• Gene Symbol: LGR6
• Gene ID (NCBI): 59352
• RRID: AB_2135163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LGR6 (Leucine-rich repeat-containing G-protein coupled receptor 6) is a seven-transmembrane protein. LGR6 is a high affinity receptor of R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. It potentially functions as a tumor suppressor. (PMID: 20223988; 20417836; 22615920)

Publications

Species	Application	Title
human		J Clin Invest; Maresin 1 activates LGR6 receptor promoting phagocyte immunoresolvent functions.; Authors - Nan Chiang ; KD Validated
human	WB	Int J Biol Macromol; Study on the mechanism of macrophages activated by phosphoesterified rehmanniae polysaccharide on human gastric cancer cells; Authors - Xianglong Liu
human	WB	Mol Ther Oncolytics; Silencing LGR6 Attenuates Stemness and Chemoresistance via Inhibiting Wnt/β-Catenin Signaling in Ovarian Cancer.; Authors - Xiaohong Ruan
human	WB	PLoS One; Characterizing the cellular attachment receptor for Langat virus.; Authors - Raquel Rodrigues
mouse	WB	JBMR Plus; Evidence for Lgr6 as a Novel Marker of Osteoblastic Progenitors in Mice.; Authors - Vikram Khedgikar
mouse	ELISA	Proc Natl Acad Sci U S A; The Maresin 1-LGR6 axis decreases respiratory syncytial virus-induced lung inflammation; Authors - Nandini Krishnamoorthy

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marc (Verified Customer) (02-14-2023)

Used this antibody on immunofluorescence, but the expression of LGR6 in the spinal cord is low so we had to perform an immunofluorescence with tyramide amplification. With tyramide amplification the result is satisfactory.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Spinal Cord