Tested Applications
| Positive WB detected in | A549 cells, HUVEC cells, mouse testis tissue, HT-29 cells, HeLa cells, U-87 MG cells, LNCaP cells, PC-3 cells |
| Positive IHC detected in | human testis tissue, human spleen tissue, human ovary tissue, mouse testis tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17658-1-AP targets LGR6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11909 Product name: Recombinant human LGR6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC047905 Sequence: MGRPRLTLVCQVSIIISARDLSMNNLTELQPGLFHHLRFLEELRLSGNHLSHIPGQAFSGLYSLKILMLQNNQLGGIPAEALWELPSLQSLRLDANLISLVPERSFEGLSSLRHLWLDDNALTEIPVRALNNLPALQAMTLALNRISHIPDYAFQNLTSLVVLHLHNNRIQHLGTHSFEGLHNLETLDLNYNKLQEFPVAIRTLGRLQELGFHNNNIKAIPEKAFMGNPLLQTIHFYDNPIQFVGRSAFQYLPKLHTLSLNGAMDIQEFPDLKGTTSLEILTLTRAGIRLLPSGMCQQLPRLRVLELSHNQIEELPSLHRCQKLEEIGLQHNRIWEIGADTFSQLSSLQAL Predict reactive species |
| Full Name | leucine-rich repeat-containing G protein-coupled receptor 6 |
| Calculated Molecular Weight | 915 aa, 99 kDa |
| Observed Molecular Weight | 90-104 kDa |
| GenBank Accession Number | BC047905 |
| Gene Symbol | LGR6 |
| Gene ID (NCBI) | 59352 |
| RRID | AB_2135163 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBX8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LGR6 (Leucine-rich repeat-containing G-protein coupled receptor 6) is a seven-transmembrane protein. LGR6 is a high affinity receptor of R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. It potentially functions as a tumor suppressor. (PMID: 20223988; 20417836; 22615920)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LGR6 antibody 17658-1-AP | Download protocol |
| WB protocol for LGR6 antibody 17658-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LGR6 Polyclonal antibody (Cat# 17658-1-AP) has 15 citations published in journals including J Clin Invest, PNAS, Biomaterials, Cell Death & Disease, J Transl Med, Int J Biol Macromol, Mol Med, Front Immunol, Mol Ther Oncolytics, Aging, Pharmacol Res, Mar Drugs, JBMR Plus, and PLoS One. Research fields span immunology/resolution of inflammation, cancer (colorectal, gastric, ovarian, prostate, glioma), wound healing, stem cell biology, viral infection, and bone biology.
| Species | Application | Title |
|---|---|---|
J Clin Invest Maresin 1 activates LGR6 receptor promoting phagocyte immunoresolvent functions.
| ||
Biomaterials Axolotl skin mechanics-biomimetic hydrogel promotes scarless wound healing associated with macrophage polarisation via Piezo1/YAP signalling. | ||
Cell Death Dis Loss of cadherin 17 downregulates LGR5 expression, stem cell properties and drug resistance in metastatic colorectal cancer cells.
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Pharmacol Res Targeted MSC therapy restores implantation by spatially reconstituting the RSPO1 niche via IL10-CXCL12 signaling. | ||
Aging (Albany NY) Comprehensive analysis of cuproptosis-related immune biomarker signature to enhance prognostic accuracy in gastric cancer | ||
J Transl Med LGR6 modulates intervertebral disc degeneration through regulation of macrophage efferocytosis
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Reviews
What customers say
The single review rates this antibody 5 stars for immunofluorescence on spinal cord tissue at a 1:500 dilution. The reviewer noted that LGR6 expression in spinal cord is low, requiring tyramide signal amplification to achieve satisfactory results. Overall, the antibody performed well when used with appropriate amplification for low-expression targets.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marc (Verified Customer) (02-14-2023) | Used this antibody on immunofluorescence, but the expression of LGR6 in the spinal cord is low so we had to perform an immunofluorescence with tyramide amplification. With tyramide amplification the result is satisfactory.
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