Tested Applications
| Positive WB detected in | mouse ovary tissue |
| Positive IHC detected in | human ovary cancer tissue, human ovary tissue, human thyroid cancer tissue, mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse ovary tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26424-1-AP targets LHCGR in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19259 Product name: Recombinant human LHCGR protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 49-219 aa of BC157028 Sequence: AGLTRLSLAYLPVKVIPSQAFRGLNEVIKIEISQIDSLERIEANAFDNLLNLSEILIQNTKNLRYIEPGAFINLPRLKYLSICNTGIRKFPDVTKVFSSESNFILEICDNLHITTIPGNAFQGMNNESVTLKLYGNGFEEVQSHAFNGTTLTSLELKENVHLEKMHNGAFR Predict reactive species |
| Full Name | LHCGR |
| Calculated Molecular Weight | 699 aa, 79 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC157028 |
| Gene Symbol | LHCGR |
| Gene ID (NCBI) | 3973 |
| RRID | AB_2880511 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22888 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LHCGR, also known as LHR or LSHR, belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs) and plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LHCGR antibody 26424-1-AP | Download protocol |
| IHC protocol for LHCGR antibody 26424-1-AP | Download protocol |
| WB protocol for LHCGR antibody 26424-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LHCGR Polyclonal antibody (Cat# 26424-1-AP) has 3 total citations. These appear in Biology of Reproduction, Biochemical and Biophysical Research Communications, and Biological Research. The associated research fields include reproductive endocrinology (testicular macrophage testosterone production, ovine corpus luteum luteolysis) and neurodegeneration (Parkinson's disease dopamine neuronal death). Cited applications include WB and IF in mouse species.
| Species | Application | Title |
|---|---|---|
Biol Res Mouse testicular macrophages can independently produce testosterone and are regulated by Cebpb | ||
Biochem Biophys Res Commun Effects of hCG on DA neuronal death of Parkinson's disease
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