LIMK1 Monoclonal antibody

• Featured Product
• KD/KO Validated

LIMK1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse, rabbit, pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2B11E9

Cat no : 67974-1-Ig

Synonyms

LIM domain kinase 1, LIMK, LIMK 1, LIMK1

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Validation Data Gallery

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  WB analysis using 67974-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis using 67974-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HeLa using 67974-1-Ig

  WB result of LIMK1 antibody (67974-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIMK1 transfected HeLa cells.

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  WB analysis of SH-SY5Y using 67974-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67974-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67974-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LIMK1 antibody (67974-1-Ig, Clone: 2B11E9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, SH-SY5Y cells, pig brain tissue, A431 cells, HEK-293 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67974-1-Ig targets LIMK1 in WB, IF, ELISA applications and shows reactivity with Human, rat, mouse, rabbit, pig samples.

• Tested Reactivity: Human, rat, mouse, rabbit, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: LIMK1 fusion protein Ag28862
• Full Name: LIM domain kinase 1
• Calculated Molecular Weight: 647 aa, 73 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC152982
• Gene Symbol: LIMK1
• Gene ID (NCBI): 3984
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIMK1,also named LIMK, belongs to the protein kinase superfamily and TKL Ser/Thr protein kinase family. LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. LIMK1 may be involved in brain development. The antibody has no cross-reaction with LIMK2.