LRCH1 Polyclonal antibody, PBS Only

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• LRCH1 Polyclonal antibody, PBS Only (25017-1-PBS)

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  WB analysis of HeLa using 25017-1-AP (same clone as 25017-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 25017-1-AP (LRCH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25017-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 25017-1-AP (same clone as 25017-1-PBS)

  IP result of anti-LRCH1 (IP:25017-1-AP, 4ug; Detection:25017-1-AP 1:300) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 25017-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 25017-1-AP (same clone as 25017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25017-1-AP (LRCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25017-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 25017-1-AP (same clone as 25017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25017-1-AP (LRCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25017-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 25017-1-AP (same clone as 25017-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using LRCH1 antibody (25017-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25017-1-PBS in a different storage buffer formulation.

LRCH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 25017-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Neuronal protein 81, Leucine-rich repeat and calponin homology domain-containing protein 1, KIAA1016, CHDC1, Calponin homology domain-containing protein 1

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Product Information

25017-1-PBS targets LRCH1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18728

  Product name: Recombinant human LRCH1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 353-549 aa of BC112937

  Sequence: NIMEEEQIIKEDSCHRLSPVKGEFHQEFQPEPSLLGDSTNSGEERDQFTDRADGLHSEFMNYKARAEDCEELLRIEEDVHWQTEGIISSSKDQDMDIAMIEQLREAVDLLQDPNGLSTDITERSVLNLYPMGSAEALELQDSALNGQIQLETSPVCEVQSDLTLQSNGSQYSPNEIRENSPAVSPTTNSTAPFGLKP

• Full Name: leucine-rich repeats and calponin homology (CH) domain containing 1
• Calculated Molecular Weight: 728 aa, 80 kDa
• Observed Molecular Weight: 85-95 kDa
• GenBank Accession Number: BC112937
• Gene Symbol: LRCH1
• Gene ID (NCBI): 23143
• RRID: AB_2879848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y2L9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LRCH1, also named as CHDC1 or Neuronal protein 81, is a 728 amino acid protein, which contains one CH domain and nine LRR repeats. The molecular weight of LRCH1 is 81 kDa and it contains phosphorylation modified residues. we detected a 85-95 kDa protein by western blot. The function of LRCH1 is still non-known.