Anti-Human LRP1 (5A6) Mouse IgG2a Recombinant Antibody

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  FC experiment of U-87 MG using 65594-1-MR

  1x10^6 U-87 MG cells were surface stained with 0.25 ug Anti-Human LRP1 (5A6) Mouse IgG2a RecAb (65594-1-MR, Clone: 5A6) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed.

LRP1 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 65594-1-MR

Clone No.5A6

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

5A6, A2MR, Alpha 2 macroglobulin receptor, Alpha-2-macroglobulin receptor, APOER

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Tested Applications

• Positive FC detected in: U-87 MG cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65594-1-MR targets LRP1 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Full length native protein (purified)
• Full Name: low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor)
• Calculated Molecular Weight: 505 kDa
• GenBank Accession Number: BC045107
• Gene Symbol: LRP1
• Gene ID (NCBI): 4035
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

LRP1 (Prolow-density lipoprotein receptor-related protein 1), also known as A2MR, APOER and CD91, is a type I transmembrane protein that belongs to the LDLR family. LRP1 is synthesized as a transmembrane glycosylated precursor of 600 kDa, and is subsequently cleaved to generate a large 515-kDa N-terminal extracellular subunit and an 85-kDa C-terminal transmembrane subunit, which are noncovalently associated with one another (PMID: 2112085; 8546712). LRP1 is a multifunctional receptor involved in the clearance of a large number of diverse ligands and modulating various cellular processes. LRP1 is implicated in conditions such as atherosclerosis, cancer and neurodegenerative diseases (PMID: 28381441).