LRRC32 Recombinant antibody, PBS Only (Capture)

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  WB analysis of HUVEC using 86059-2-RR (same clone as 86059-2-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 86059-2-RR (LRRC32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86059-2-PBS in a different storage buffer formulation.

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  WB analysis of human peripheral blood platelets using 86059-2-RR (same clone as 86059-2-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 86059-2-RR (LRRC32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86059-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02335-1

  Sandwich ELISA standard curve of MP02335-1, Human LRRC32 Recombinant Matched Antibody Pair - PBS only. 86059-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag23423. 86059-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP02335-1

  The mean LRRC32 concentration was determined to be 16.61 ng/mL in human placenta tissue extract based on a 3.2 mg/mL extract load.

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  Sample test of MP02335-1

  Serum of sixteen individual healthy human donors was measured. The human LRRC32 concentration of detected samples was determined to be 0.37 ng/mL with a range of 0 - 1.01 ng/mL.

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  Affinity and Kinetic Characterization of 86059-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86059-2-RR against Human LRRC32 were performed. The affinity constant is 1.40 nM.

LRRC32 Uni-rAb^TM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 86059-2-PBS

Clone No.250611B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Garpin, D11S833E, GARP, Leucine-rich repeat-containing protein 32, Transforming growth factor beta activator LRRC32

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Product Information

86059-2-PBS targets LRRC32 as part of a matched antibody pair:

MP02335-1: 86059-2-PBS capture and 86059-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LRRC32 fusion protein Ag23423
• Full Name: leucine rich repeat containing 32
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 72-80 kDa
• GenBank Accession Number: BC070079
• Gene Symbol: LRRC32
• Gene ID (NCBI): 2615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14392
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LRRC32, also known as GARP, is a transmembrane protein of 662 amino acids, the extracellular portion of which contains 20 leucine-rich repeats (PMID: 8180135). LRRC32 is a cell surface receptor on regulatory T-lymphocytes (Treg cells), platelets, hepatic stellate cells and certain cancer cells (PMID: 27054568). It has been demonstrated as a Treg-specific activation marker (PMID: 20137067). LRRC32 is critical for the surface expression of latent TGF-β by binding to the complex and functioning as its cell surface receptor (PMID: 19651619).