LRRC32 Recombinant antibody

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  WB analysis of HUVEC using 86059-2-RR

  HUVEC cells were subjected to SDS PAGE followed by western blot with 86059-2-RR (LRRC32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood platelets using 86059-2-RR

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 86059-2-RR (LRRC32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86059-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86059-2-RR against Human LRRC32 were performed. The affinity constant is 1.40 nM.

LRRC32 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86059-2-RR

Clone No.250611B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Garpin, D11S833E, GARP, Leucine-rich repeat-containing protein 32, Transforming growth factor beta activator LRRC32

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Tested Applications

• Positive WB detected in: human peripheral blood platelets, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86059-2-RR targets LRRC32 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LRRC32 fusion protein Ag23423
• Full Name: leucine rich repeat containing 32
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 72-80 kDa
• GenBank Accession Number: BC070079
• Gene Symbol: LRRC32
• Gene ID (NCBI): 2615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14392
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRRC32, also known as GARP, is a transmembrane protein of 662 amino acids, the extracellular portion of which contains 20 leucine-rich repeats (PMID: 8180135). LRRC32 is a cell surface receptor on regulatory T-lymphocytes (Treg cells), platelets, hepatic stellate cells and certain cancer cells (PMID: 27054568). It has been demonstrated as a Treg-specific activation marker (PMID: 20137067). LRRC32 is critical for the surface expression of latent TGF-β by binding to the complex and functioning as its cell surface receptor (PMID: 19651619).