LSM12 Monoclonal antibody

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  WB analysis using 67029-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67029-1-Ig (LSM12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67029-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67029-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67029-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67029-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67029-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 67029-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug LSM12 Monoclonal antibody (67029-1-Ig, Clone:2E3G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

LSM12 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 67029-1-Ig

Clone No.2E3G3

Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

Protein LSM12, 2E3G3

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

67029-1-Ig targets LSM12 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28782

  Product name: Recombinant human LSM12 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-195 aa of BC044587

  Sequence: MAAPPGEYFSVGSQVSCRTCQEQRLQGEVVAFDYQSKMLALKCPSSSGKPNHADILLINLQYVSEVEIINDRTETPRPLASLNVSKLASKARTEKEEKLSQAYAISAGVSLEGQQLFQTIHKTIKDCKWQEKNIVVMEEVVITPPYQVENCKGKEGSALSHVRKIVEKHFRDVESQKILQRSQAQQPQKEAALSS

• Full Name: LSM12 homolog (S. cerevisiae)
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC044587
• Gene Symbol: LSM12
• Gene ID (NCBI): 124801
• RRID: AB_2882344
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q3MHD2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Signal; Convergent activation of Ca2+ permeability in two-pore channel 2 through distinct molecular routes; Authors - Ryo Saito ; KO Validated