LSM12 Monoclonal antibody, PBS Only

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  WB analysis using 67029-1-Ig (same clone as 67029-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67029-1-Ig (LSM12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67029-1-Ig (same clone as 67029-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug LSM12 Monoclonal antibody (67029-1-Ig, Clone:2E3G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

LSM12 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 67029-1-PBS

Clone No.2E3G3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

Protein LSM12, 2E3G3

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Product Information

67029-1-PBS targets LSM12 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28782

  Product name: Recombinant human LSM12 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-195 aa of BC044587

  Sequence: MAAPPGEYFSVGSQVSCRTCQEQRLQGEVVAFDYQSKMLALKCPSSSGKPNHADILLINLQYVSEVEIINDRTETPRPLASLNVSKLASKARTEKEEKLSQAYAISAGVSLEGQQLFQTIHKTIKDCKWQEKNIVVMEEVVITPPYQVENCKGKEGSALSHVRKIVEKHFRDVESQKILQRSQAQQPQKEAALSS

• Full Name: LSM12 homolog (S. cerevisiae)
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC044587
• Gene Symbol: LSM12
• Gene ID (NCBI): 124801
• RRID: AB_2882344
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q3MHD2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.