Anti-Human M-CSF Rabbit Recombinant Antibody

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• Anti-Human M-CSF Rabbit Recombinant Antibody (98756-2-RR)

M-CSF Uni-rAb^® Recombinant Antibody for IHC, FC (Intra)

Cat No. 98756-2-RR

Clone No.252446E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC (Intra)

Synonyms

CSF 1, CSF1, CSF-1, Lanimostim, M CSF

• 

  IHC staining of human tonsillitis using 98756-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98756-2-RR (M-CSF antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 98756-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98756-2-RR (M-CSF antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of MG-63 using 98756-2-RR

  1x10^6 MG-63 cells were intracellularly stained with 0.25 ug Anti-Human M-CSF Rabbit RecAb (98756-2-RR, Clone: 252446E3) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: MG-63 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98756-2-RR targets M-CSF in IHC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo:

  Product name: HumanKine® recombinant human M-CSF protein

  Source: -derived,

  Tag:

  Domain: of

  Sequence:

• Full Name: colony stimulating factor 1 (macrophage)
• Gene Symbol: M-CSF
• Gene ID (NCBI): 1435
• RRID: AB_3746323
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09603
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance. (PMID: 29734839; 29679908; 28202039)