CoraLite® Plus 488 Anti-Human M-CSF Rabbit Recombinant Antibody

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• CoraLite® Plus 488 Anti-Human M-CSF Rabbit Recombinant Antibody (CL488-98756-2)

M-CSF Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL488-98756-2 Copied!

Clone No. 252446E3

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

CSF 1, CSF1, CSF-1, Lanimostim, M CSF

• 

  FC experiment of MG-63 using CL488-98756-2

  1x10^6 MG-63 cells were intracellularly stained with 0.25 ug / 5 ul CoraLite® Plus 488 Anti-Human M-CSF Rabbit RecAb (CL488-98756-2, Clone: 252446E3) (red) or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: MG-63 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-98756-2 targets M-CSF in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo:

  Product name: HumanKine® recombinant human M-CSF protein

  Source: -derived,

  Tag:

  Domain: of

  Sequence:

• Full Name: colony stimulating factor 1 (macrophage)
• Gene Symbol: M-CSF
• Gene ID (NCBI): 1435
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09603
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance. (PMID: 29734839; 29679908; 28202039)