CoraLite® Plus 488-conjugated M-cadherin Recombinant antibody

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  IF Staining of H9C2 using CL488-84440-5

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using CoraLite® Plus 488 M-cadherin antibody (CL488-84440-5, Clone: 241837C10 ) at dilution of 1:200.

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  IF Staining of C2C12 using CL488-84440-5

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CoraLite® Plus 488 M-cadherin antibody (CL488-84440-5, Clone: 241837C10 ) at dilution of 1:200.

M-cadherin Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-84440-5

Clone No.241837C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

CDH15, 241837C10, Cadherin 14, Cadherin 15, Cadherin-14

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Tested Applications

• Positive IF/ICC detected in: H9C2 cells, C2C12 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84440-5 targets M-cadherin in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: M-cadherin fusion protein Ag25763
• Full Name: cadherin 15, type 1, M-cadherin (myotubule)
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 90-130 kDa
• GenBank Accession Number: BC008951
• Gene Symbol: M-cadherin
• Gene ID (NCBI): 1013
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P55291
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. M-cadherin (muscle cadherin), also known as CDH15 (cadherin-15), is a 124-kDa type I transmembrane glycoprotein that is highly expressed in developing skeletal muscle, satellite cells, and cerebellum (PMID: 9545347; 12052883). It associates with β-catenin and functions as a cell-cell adhesion molecule in a homophilic and specific manner (PMID: 9545347). M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation (PMID: 1840697).