MAD2L2 Monoclonal antibody

MAD2L2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2A4C2

Cat no : 67100-1-Ig

Synonyms

hREV7, MAD2 like protein 2, MAD2B, MAD2L2, REV7, REV7 homolog



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67100-1-Ig targets MAD2L2 in WB, IHC,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MAD2L2 fusion protein Ag28233
Full Name MAD2 mitotic arrest deficient-like 2 (yeast)
Calculated molecular weight 211 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC015244
Gene symbol MAD2L2
Gene ID (NCBI) 10459
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.

Protocols

Product Specific Protocols
WB protocol for MAD2L2 antibody 67100-1-IgDownload protocol
IHC protocol for MAD2L2 antibody 67100-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols