MAD2L2 Monoclonal antibody

MAD2L2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b


human, mouse, rat


WB, IHC, FC (Intra), ELISA





Cat no : 67100-1-Ig


Mitotic arrest deficient 2-like protein 2, MAD2-like protein 2, MAD2B, MAD2 like protein 2, hREV7

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inK-562 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67100-1-Ig targets MAD2L2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MAD2L2 fusion protein Ag28233
Full Name MAD2 mitotic arrest deficient-like 2 (yeast)
Calculated Molecular Weight 211 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC015244
Gene Symbol MAD2L2
Gene ID (NCBI) 10459
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.


Product Specific Protocols
WB protocol for MAD2L2 antibody 67100-1-IgDownload protocol
IHC protocol for MAD2L2 antibody 67100-1-IgDownload protocol
FC protocol for MAD2L2 antibody 67100-1-IgDownload protocol
Standard Protocols
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