MAOA Monoclonal antibody

MAOA Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A9E4

Cat no : 68471-1-Ig

Synonyms

MAO A, MAOA, monoamine oxidase A, Monoamine oxidase type A

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Validation Data Gallery

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  WB analysis using 68471-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68471-1-Ig (MAOA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 68471-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 68471-1-Ig (MAOA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 68471-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68471-1-Ig (MAOA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 68471-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68471-1-Ig (MAOA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 68471-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68471-1-Ig (MAOA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, U2OS cells, LNCaP cells, Jurkat cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68471-1-Ig targets MAOA in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAOA fusion protein Ag30028
• Full Name: monoamine oxidase A
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC008064
• Gene Symbol: MAOA
• Gene ID (NCBI): 4128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAOA belongs to the flavin monoamine oxidase family. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.