CoraLite® Plus 488-conjugated MAP2 Recombinant antibody

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  IF Staining of rat brain using CL488-84306-4

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite® Plus 488 MAP2 antibody (CL488-84306-4, Clone: 241653H8 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MAP2 Uni-rAb^TM Recombinant Antibody for IF-P

Cat No. CL488-84306-4

Clone No.241653H8

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF-P

Synonyms

241653H8, MAP 2, MAP-2, MAP2A, MAP2B

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Tested Applications

• Positive IF-P detected in: rat brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84306-4 targets MAP2 in IF-P applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MAP2 fusion protein Ag11580
• Full Name: microtubule-associated protein 2
• Calculated Molecular Weight: 200 kDa
• Observed Molecular Weight: 280-300 kDa, 70-85 kDa
• GenBank Accession Number: BC038857
• Gene Symbol: MAP2
• Gene ID (NCBI): 4133
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11137
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in the brain and has an important role in neuronal morphogenesis. Multiple high MW and low MW MAP2 isoforms are expressed within the proximal segment of axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally-specific manner. The 280 kDa MAP2B is present throughout rat brain development, and the slightly larger MAP2A appears first during the end of the second week of postnatal life. MAP2C, composed of several bands of about 70 kDa, is present during early brain development and largely disappears from the mature brain except for the retina, olfactory bulb, and cerebellum. In addition, some isoforms with lower MW around 50-60 kDa also exist. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.