MAP2K4 Monoclonal antibody, PBS Only

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  WB analysis using 67333-1-Ig (same clone as 67333-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67333-1-Ig (MAP2K4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67333-1-Ig (same clone as 67333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67333-1-Ig (same clone as 67333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67333-1-Ig (same clone as 67333-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAP2K4 antibody (67333-1-Ig, Clone: 2E4C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

MAP2K4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67333-1-PBS

Clone No.2E4C1

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MEK4, 2E4C1, c-Jun N-terminal kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 4, EC:2.7.12.2

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Product Information

67333-1-PBS targets MAP2K4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11348

  Product name: Recombinant human MAP2K4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 50-399 aa of BC036032

  Sequence: NPPFKSTARFTLNPNPTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDLKDLGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPYIVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATPSSPMYVD

• Full Name: mitogen-activated protein kinase kinase 4
• Calculated Molecular Weight: 399 aa, 44 kDa
• Observed Molecular Weight: 44-50 kDa
• GenBank Accession Number: BC036032
• Gene Symbol: MAP2K4
• Gene ID (NCBI): 6416
• RRID: AB_2882591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P45985
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MEK4 is also named as JNKK1, MAP2K4 , MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MEK4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.