|Positive WB detected in||HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
67333-1-Ig targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||MAP2K4 fusion protein Ag11348|
|Full Name||mitogen-activated protein kinase kinase 4|
|Calculated molecular weight||399 aa, 44 kDa|
|Observed molecular weight||44-50 kDa|
|GenBank accession number||BC036032|
|Gene ID (NCBI)||6416|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MEK4 is also named as JNKK1, MAP2K4 , MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MEK4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.
A potent tumor-selective ERK pathway inactivator with high therapeutic index.
Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity