CoraLite®594-conjugated MAP2K4 Monoclonal antibody

MAP2K4 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E4C1

Cat no : CL594-67333

Synonyms

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-67333

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 MAP2K4 antibody (CL594-67333, Clone: 2E4C1 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL594-67333

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MAP2K4 (CL594-67333, Clone:2E4C1) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67333 targets MAP2K4 in IF, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAP2K4 fusion protein Ag11348
• Full Name: mitogen-activated protein kinase kinase 4
• Calculated Molecular Weight: 399 aa, 44 kDa
• Observed Molecular Weight: 44-50 kDa
• GenBank Accession Number: BC036032
• Gene Symbol: MAP2K4
• Gene ID (NCBI): 6416
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEK4 is also named as JNKK1, MAP2K4 , MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MEK4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.