MARVELD2 Recombinant antibody

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  WB analysis of HeLa using 86188-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86188-1-RR (MARVELD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 86188-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MARVELD2 antibody (86188-1-RR, Clone: 250774G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MARVELD2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86188-1-RR

Clone No.250774G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

DFNB49, MARVD2, MARVEL domain containing 2, MARVEL domain-containing protein 2, MRVLDC2

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86188-1-RR targets MARVELD2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MARVELD2 fusion protein Ag4446
• Full Name: MARVEL domain containing 2
• Calculated Molecular Weight: 558 aa, 64 kDa
• Observed Molecular Weight: 60-64 kDa
• GenBank Accession Number: BC033689
• Gene Symbol: MARVELD2
• Gene ID (NCBI): 153562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N4S9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARVELD2, also named as Tricellulin or TRIC, is a 558 amino acid protein, which contains one MARVEL domain. MARVELD2 localizes the cell membrane and plays a role in the formation of the epithelial barriers. MARVELD2, is a first integral membrane protein that is concentrated at the vertically oriented tight junction strands of tricellular contacts. MARVELD2 may be a component to maintain the integrity for peripheral nervous system myelin function and morphology.