Tested Applications
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, HeLa cells |
| Positive ChIP-qPCR detected in | HEK293 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82852-3-RR targets MAZ in IHC, IF/ICC, ELISA, ChIP-qPCR applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag15200 Product name: Recombinant human MAZ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC041629 Sequence: MVPLSLLSVPQLSGAGGGGGEAGAGGGAAAVAAGGVVTTTASGKRIRKNHACEMCGKAFRDVYHLNRHKLSHSDEKPYQCPVCQQRFKRKDRMSYHVRSHDGAVHKPYNCSHCGKSFSRPDHLNSHVRQVHSTERPFKCEKCEAAFATKDRLRAHTVRHEEKVPCHVCGKMLSSAYISDHMKVHSQGPHHVCELCNKG Predict reactive species |
| Full Name | MYC-associated zinc finger protein (purine-binding transcription factor) |
| Calculated Molecular Weight | 477 aa, 49 kDa |
| Observed Molecular Weight | 49-51 kDa |
| GenBank Accession Number | BC041629 |
| Gene Symbol | MAZ |
| Gene ID (NCBI) | 4150 |
| RRID | AB_3086554 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P56270 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Myc-associated zinc-finger protein (MAZ) is a transcription factor with dual roles in transcription initiation and termination. MAZ can activate the expression of a variety of genes such as the c-Myc, insulin, VEGF, and RAS gene family and the serotonin 1A receptor. MAZ plays a critical role in the progression of various cancers including breast, prostate, liver, and so on. Deregulation of MAZ expression is associated with the progression of pancreatic ductal adenocarcinoma (PDAC)(PMID: 29414775).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MAZ antibody 82852-3-RR | Download protocol |
| IHC protocol for MAZ antibody 82852-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MAZ Recombinant monoclonal antibody (1D5), catalog number 82852-3-RR, has 1 citation. It appears in Communications Biology (IF 5.8), published in November 2024. The cited study investigates the molecular mechanisms by which MAZ up-regulates NDUFS3 expression to promote malignant progression in melanoma, placing this antibody within the research fields of cancer biology and melanoma pathogenesis. The application used was IHC on human samples.
| Species | Application | Title |
|---|---|---|
Commun Biol Molecular mechanisms of MAZ targeting up-regulation of NDUFS3 expression to promote malignant progression in melanoma |
