ChromoTek MBP-Trap Agarose beads, kit

The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.

Specificity

Maltose-binding protein (MBP)

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : mbtak

Synonyms

MBP, Maltose binding protein, MBP-Trap



Product Information

The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
Clonality VHH
HostAlpaca
AffinityDissociation constant KD of 4 nM
Specificity/TargetMaltose-binding protein (MBP) of Escherichia coli
Coupled VHHRecombinant, monoclonal anti-MBP single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4% agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
MBP-Trap Agarose beads20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution