ChromoTek MBP-Trap Agarose beads, kit

The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.

Specificity

Maltose-binding protein (MBP)

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : mbtak

Synonyms

MBP, Maltose binding protein, MBP-Trap

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Validation Data Gallery

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  Immunoprecipitation of maltose binding protein (MBP) from E. coli cell extract. The Western blot shows the very high effectivity of the MBP-Trap: No MBP is left in Flow-Through lane. I: Input, FT: Flow-Through, B: Bound.

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Product Information

The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: VHH
• Host: Alpaca
• Affinity: Dissociation constant KD of 4 nM
• Specificity/Target: Maltose-binding protein (MBP) of Escherichia coli
• Coupled VHH: Recombinant, monoclonal anti-MBP single domain antibody (sdAb) fragment
• Conjugate: Agarose
• Beads: ~ 90 µm (cross-linked 4% agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Storage Buffer: 20% EtOH
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Kit components

Component	Description
MBP-Trap Agarose beads	20 reactions (500 µL)
Lysis buffer	Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.
Dilution buffer	Dilution of cell lysate
Elution buffer	For acidic elution