ChromoTek MBP-Trap Agarose beads, kit
The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.
Specificity
Maltose-binding protein (MBP)
Applications
IP, CoIP, ChIP, RIP
Conjugate
Agarose
Cat no : mbtak
Synonyms
Validation Data Gallery
Product Information
The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 4 nM |
Specificity/Target | Maltose-binding protein (MBP) of Escherichia coli |
Coupled VHH | Recombinant, monoclonal anti-MBP single domain antibody (sdAb) fragment |
Conjugate | Agarose |
Beads | ~ 90 µm (cross-linked 4% agarose beads) |
Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 20% EtOH |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Kit components
Component | Description |
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MBP-Trap Agarose beads | 20 reactions (500 µL) |
Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
Wash buffer | Removal of unwanted proteins, peptides, etc. |
Dilution buffer | Dilution of cell lysate |
Elution buffer | For acidic elution |
Documentation
SDS |
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SDS MBP-Trap Agarose Kit (PDF) |
Protocol |
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Protocol MBP-Trap Agarose Kit (PDF) |
Whitepaper |
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Immunoprecipitation of GST- and MBP-fusion proteins (PDF) |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (PDF) |