ChromoTek MBP-Trap Agarose, Kit for Immunoprecipitation

Validation Data Gallery

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  Immunoprecipitation of maltose binding protein (MBP) from E. coli cell extract. The Western blot shows the very high effectivity of the MBP-Trap: No MBP is left in Flow-Through lane.I: Input, FT: Flow-Through, B: Bound.

The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.

Cat No. mbtak

Specificity

MBP

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Agarose

Synonyms

MBP, Maltose binding protein, MBP-Trap

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Product Information

The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.

• Description: The MBP-Trap Agarose Kit contains MBP-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of MBP-fusion proteins and their interacting factors.

  • Immunoprecipitation of low abundant MBP-fusion proteins

  • Significantly higher sensitivity than amylose resin and other affinity resins

  • No heavy & light antibody chains

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: Maltose-binding protein (MBP) of Escherichia coli
• Binding capacity: 45 μg (0.625 nmol) of recombinant MBP-tagged protein (~74.2 kDa) per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 2 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 4 nM
• Compatibility with mass spectrometry: The MBP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2631382
• Storage Buffer: 20% EtOH
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Kit components

• Component: Description
• MBP-Trap Agarose beads: 20 reactions (500 µL)
• Lysis buffer: Optimized for cytoplasmatic proteins and mammalian cell lysis
• RIPA buffer: Optimized for nuclear/chromatin proteins and mammalian cell lysis
• Wash buffer: Removal of unwanted proteins, peptides, etc.
• Dilution buffer: Dilution of cell lysate
• Elution buffer: For acidic elution

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