ChromoTek MBP-Trap Agarose beads

The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.

Specificity

Maltose-binding protein (MBP)

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Publications

9

Cat no : mbta

Synonyms

MBP, Maltose binding protein, MBP-Trap

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Validation Data Gallery

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  Immunoprecipitation of maltose binding protein (MBP) from E. coli cell extract. The Western blot shows the very high effectivity of the MBP-Trap: No MBP is left in Flow-Through lane.I: Input, FT: Flow-Through, B: Bound.

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Product Information

The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 4 nM
• Specificity/Target: Maltose-binding protein (MBP) of Escherichia coli
• Coupled VHH: Recombinant, monoclonal anti-MBP single domain antibody (sdAb) fragment
• Conjugate: Agarose
• Beads: ~ 90 µm (cross-linked 4% agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Storage Buffer: 20% EtOH
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Publications

Application	Title
IP	Sci Rep; Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis.; Authors - Tanja Bartoschik
IP	J Biol Chem; The RNA-binding protein FUS is chaperoned and imported into the nucleus by a network of import receptors.; Authors - Imke Baade
IP	J Cell Sci; Fission yeast Opy1 is an endogenous PI(4,5)P2 sensor that binds to the phosphatidylinositol 4-phosphate 5-kinase Its3.; Authors - Chloe E Snider
IP	Structure; Structural Basis of CYRI-B Direct Competition with Scar/WAVE Complex for Rac1.; Authors - Tamas Yelland
IP	Elife; Ceapins block the unfolded protein response sensor ATF6α by inducing a neomorphic inter-organelle tether.; Authors - Sandra Elizabeth Torres
IP	J Cell Biol; CYRI-A limits invasive migration through macropinosome formation and integrin uptake regulation.; Authors - Anh Hoang Le