ChromoTek MBP-Trap Agarose beads
The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.
Specificity
Maltose-binding protein (MBP)
Applications
IP, CoIP, ChIP, RIP
Conjugate
Agarose
Cat no : mbta
Synonyms
Validation Data Gallery
Product Information
The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.
| Applications | IP, CoIP, ChIP, RIP |
| Clonality | Monoclonal |
| Host | Alpaca |
| Affinity | Dissociation constant KD of 4 nM |
| Specificity/Target | Maltose-binding protein (MBP) of Escherichia coli |
| Coupled VHH | Recombinant, monoclonal anti-MBP single domain antibody (sdAb) fragment |
| Conjugate | Unconjugated |
| Beads | ~ 90 µm (cross-linked 4% agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Storage Buffer | 20% EtOH |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
| SDS |
|---|
| SDS MBP-Trap Agarose (PDF) |
| Protocol |
|---|
| Protocol MBP-Trap Agarose (PDF) |
| Trouble shooting |
|---|
| Troubleshooting guide immunoprecipitation (PDF) |
| Whitepaper |
|---|
| Immunoprecipitation of GST- and MBP-fusion proteins (PDF) |
Publications
| Application | Title |
|---|---|
Sci Rep Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis. | |
J Biol Chem The RNA-binding protein FUS is chaperoned and imported into the nucleus by a network of import receptors. | |
J Cell Sci Fission yeast Opy1 is an endogenous PI(4,5)P2 sensor that binds to the phosphatidylinositol 4-phosphate 5-kinase Its3. | |
Elife Ceapins block the unfolded protein response sensor ATF6α by inducing a neomorphic inter-organelle tether. | |
J Cell Biol CYRI-A limits invasive migration through macropinosome formation and integrin uptake regulation. |