MED6 Polyclonal antibody, PBS Only

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• MED6 Polyclonal antibody, PBS Only (15338-1-PBS)

• 

  WB analysis using 15338-1-AP (same clone as 15338-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15338-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 15338-1-PBS

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 15338-1-AP (same clone as 15338-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15338-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 15338-1-AP (same clone as 15338-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15338-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 15338-1-AP (same clone as 15338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15338-1-AP (MED6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15338-1-PBS in a different storage buffer formulation.

MED6 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15338-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

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Product Information

15338-1-PBS targets MED6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7541

  Product name: Recombinant human MED6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-246 aa of BC004106

  Sequence: MAAVDIRDNLLGISWVDSSWIPILNSGSVLDYFSERSNPFYDRTCNNEVVKMQRLTLEHLNQMVGIEYILLHAQEPILFIIRKQQRQSPAQVIPLADYYIIAGVIYQAPDLGSVINSRVLTAVHGIQSAFDEAMSYCRYHPSKGYWWHFKDHEEQDKVRPKAKRKEEPSSIFQRQRVDALLLDLRQKFPPKFVQLKPGEKPVPVDQTKKEAEPIPETVKPEEKETTKNVQQTVSAKGPPEKRMRLQ

• Full Name: mediator complex subunit 6
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-32 kDa
• GenBank Accession Number: BC004106
• Gene Symbol: MED6
• Gene ID (NCBI): 10001
• RRID: AB_2142554
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75586
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.