MEF2C Polyclonal antibody

MEF2C Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 20326-1-AP

Synonyms

MEF2C, myocyte enhancer factor 2C



Tested Applications

Positive WB detected inHeLa cells, mouse small intestine tissue, mouse testis tissue, rat lymph tissue, SH-SY5Y cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20326-1-AP is MEF2C Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEF2C fusion protein Ag13255
Full Name myocyte enhancer factor 2C
Calculated molecular weight 469 aa, 51 kDa
Observed molecular weight 65-68 kDa
GenBank accession numberBC026341
Gene symbol MEF2C
Gene ID (NCBI) 4208
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. This antibody is a rabbit polyclonal antibody raised against an internal 161Aa region of human MEF2C.Phosphorylation and acetylation may affect the molecular weight of protein,and 60-70kd was also been reported(PMID:28973134).

Protocols

Product Specific Protocols
WB protocol for MEF2C antibody 20326-1-APDownload protocol
IHC protocol for MEF2C antibody 20326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols