Tested Applications
| Positive WB detected in | HeLa cells, mouse small intestine tissue, mouse testis tissue, rat lymph tissue, SH-SY5Y cells |
| Positive IHC detected in | human lung tissue, human thyroid cancer tissue, rat brain tissue, rat kidney tissue, rat skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20326-1-AP targets MEF2C in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13255 Product name: Recombinant human MEF2C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-162 aa of BC026341 Sequence: PSLQRNSMSPGVTHRPPSAGNTGGLMGGDLTSGAGTSAGNGYGNPRNSPGLLVSPGNLNKNMQAKSPPPMNLGMNNRKPDLRVLIPPGSKNTMPSVSEDVDLLLNQRINNSQSAQSLATPVVSVATPTLPGQGMGGYPSAISTTYGTEYSLSSADLSSLSGF Predict reactive species |
| Full Name | myocyte enhancer factor 2C |
| Calculated Molecular Weight | 469 aa, 51 kDa |
| Observed Molecular Weight | 45-70 kDa |
| GenBank Accession Number | BC026341 |
| Gene Symbol | MEF2C |
| Gene ID (NCBI) | 4208 |
| RRID | AB_10693772 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q06413 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MEF2C antibody 20326-1-AP | Download protocol |
| IHC protocol for MEF2C antibody 20326-1-AP | Download protocol |
| WB protocol for MEF2C antibody 20326-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this product (used in Western Blot) was rated 5 stars. The reviewer reported that the antibody was exactly what was needed for their research and that it arrived quickly, without requiring any additional QC review. Overall, the feedback is positive, highlighting both the product's suitability for the intended application and the fast, hassle-free delivery.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
ANDREW (Verified Customer) (07-20-2026) | EXACTLY WHAT WAS NEEDED FOR THE RESEARCH. ARRIVED QUICKLY WITHOUT NEEDING ANY ADDITIONAL QC REVIEW.
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