METTL11A Polyclonal antibody, PBS Only

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• METTL11A Polyclonal antibody, PBS Only (17184-1-PBS)

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  WB analysis using 17184-1-AP (same clone as 17184-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17184-1-AP (METTL11A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17184-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 17184-1-AP (same clone as 17184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17184-1-AP (METTL11A antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17184-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 17184-1-AP (same clone as 17184-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using METTL11A antibody (17184-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17184-1-PBS in a different storage buffer formulation.

METTL11A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 17184-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AD 003, AD-003, Alpha N-terminal protein methyltransferase 1A, C9orf32, EC:2.1.1.244

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Product Information

17184-1-PBS targets METTL11A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10967

  Product name: Recombinant human METTL11A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-223 aa of BC033234

  Sequence: MTSEVIEDEKQFYSKAKTYWKQIPPTVDGMLGGYGHISSIDINSSRKFLQRFLREGPNKTGTSCALDCGAGIGRITKRLLLPLFREVDMVDITEDFLVQAKTYLGEEGKRVRNYFCCGLQDFTPEPDSYDVIWIQWVIGHLTDQHLAEFLRRCKGSLRPNGIIVIKDNMAQEGVILDDVDSSVCRDLDVVRRIICSAGLSLLAEERQENLPDEIYHVYSFALR

• Full Name: methyltransferase like 11A
• Calculated Molecular Weight: 223 aa, 25 kDa
• Observed Molecular Weight: 16 kDa, 26 kDa
• GenBank Accession Number: BC033234
• Gene Symbol: METTL11A
• Gene ID (NCBI): 28989
• RRID: AB_2142020
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BV86
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

METTL11A, also known as NTMT1 (Nα-trimethyllysine hydrolase), is an enzyme that belongs to the methyltransferase-like (METTL) protein family. It plays a crucial role in epigenetic processes, particularly in the methylation of proteins, which can influence gene expression and other cellular functions. METTL11A is regulated by other members of the METTL family, such as METTL11B and METTL13, which can either activate or inhibit its function.