CoraLite®594-conjugated METTL3 Monoclonal antibody

• Featured Product
• KD/KO Validated

METTL3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D8H1

Cat no : CL594-67733

Synonyms

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8

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Validation Data Gallery

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  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:400.

• 

  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:400.

• 

  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse testis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Product Information

CL594-67733 targets METTL3 in IF applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: METTL3 fusion protein Ag7110
• Full Name: methyltransferase like 3
• Calculated molecular weight: 64 kDa
• Observed molecular weight: 65-70 kDa
• GenBank accession number: BC001650
• Gene symbol: METTL3
• Gene ID (NCBI): 56339
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 593 nm / 614 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.