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CoraLite®594-conjugated METTL3 Monoclonal antibody
METTL3 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse
Applications
IF
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2D8H1
Cat no : CL594-67733
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | mouse testis tissue |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Product Information
CL594-67733 targets METTL3 in IF applications and shows reactivity with Human, Mouse samples.
Tested Reactivity | Human, Mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | METTL3 fusion protein Ag7110 |
Full Name | methyltransferase like 3 |
Calculated molecular weight | 64 kDa |
Observed molecular weight | 65-70 kDa |
GenBank accession number | BC001650 |
Gene symbol | METTL3 |
Gene ID (NCBI) | 56339 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 593 nm / 614 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 METTL3 antibody CL594-67733 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |