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METTL4 Polyclonal antibody, PBS Only (17718-1-PBS)

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WB analysis of MCF-7 using 17718-1-AP (same clone as 17718-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 17718-1-AP (METTL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17718-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 17718-1-AP (same clone as 17718-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 17718-1-AP (METTL4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17718-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 17718-1-AP (same clone as 17718-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 17718-1-AP (METTL4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17718-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 17718-1-AP (same clone as 17718-1-PBS)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17718-1-AP (METTL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17718-1-PBS in a different storage buffer formulation.

METTL4 Polyclonal antibody, PBS Only

Name: METTL4 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 17718-1-PBS

METTL4 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 17718-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

EC:2.1.1.-, EC:2.1.1.72, HsT661, methyltransferase like 4, Methyltransferase-like protein 4

Formulation: PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

17718-1-PBS targets METTL4 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12093 Product name: Recombinant human METTL4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 125-472 aa of BC111020 Sequence: ISISIIGKKRKRCVVFNQGELDAMEYHTKIRELILDGSLQLIQEGLKSGFLYPLFEKQDKGSKPITLPLDACSLSELCEMAKHLPSLNEMEHQTLQLVEEDTSVTEQDLFLRVVENNSSFTKVITLMGQKYLLPPKSSFLLSDISCMQPLLNYRKTFDVIVIDPPWQNKSVKRSNRYSYLSPLQIKQIPIPKLAAPNCLLVTWVTNRQKHLRFIKEELYPSWSVEVVAEWHWVKITNSGEFVFPLDSPHKKPYEGLILGRVQEKTALPLRNADVNVLPIPDHKLIVSVPCTLHSHKPPLAEVLKDYIKPDGEYLELFARNLQPGWTSWGNEVLKFQHVDYFIALESGS Predict reactive species
Full Name	methyltransferase like 4
Calculated Molecular Weight	472 aa, 54 kDa
GenBank Accession Number	BC111020
Gene Symbol	METTL4
Gene ID (NCBI)	64863
RRID	AB_2142047
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N3J2
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Methyltransferase like protein 4 (METTL4) is a internal m6Am methyltransferase, which mediates N6-methylation of Am30 on U2 snRNA in the context of an AAG motif in vivo and in vitro. METTL4 belongs to the MT-A70-like family, and like its paralogs METTL3/14, METTL4 is conserved from yeast to human.